Modification of construction, optical and luminescent properties of graphene oxide (GO) by laser ablation at 532 nm throughout numerous occasions was studied. It was proven that after 30 minutes of laser ablation, the common lateral dimension of GO sheets decreases from 272±120 nm to 62±29 nm. Research of structural properties by Raman and FTIR spectroscopy and microscopy point out the discount and graphitization of GO at laser irradiation occasions larger than 20 minutes, which ends up in a rise within the common crystallite dimension of sp2 carbon domains and a lower within the variety of GO layers within the ensuing particles. The optical density of the ensuing GO options and the depth of their fluorescence are additionally time dependent. After ablation, the GO fluorescence depth elevated by 10, 25, and 36% for 10, 20 and 30 minutes, respectively. Within the long-lived luminescence spectrum of GO, a broad emission band was recorded after ablation, whereas for GO earlier than ablation long-lived luminescence was not exhibited.